Most possiblities include::
1. Stress
2. Increased exercise- some women athletes don't have periods for many months at a time.
3. Weight loss- this is true for many women losing weight, worse for sufferers of bulimia/anorexia.
4. Illness
5. Pregnancy
She's already taken two tests and the results were negative. If she wants and is worried about it she should go see a doctor. This is the best choice/decision to make. I personally would wait another couple weeks and take another over the counter test.
Professionals say to not fret over 1 or 2 missed periods unless you are concerned about pregnancy. Wait a little longer and if the periods remain few and irregular and she's still not pregnant then it's for the best she get a history and pelvic exam. She may have something healthwise going on inside that could be causing it.
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