I started picking them up for the additional story because I'm a nerd.
However, once I learned there was an achievement (achievement whore that I am lately...) and that I had already picked up 96-97% of them anyway, I went through a downloaded list and found out that I missed one I can't go back for....

45 minutes into Olympic Heights....You still have a bit, but you're almost there. If memory serves, there's Apollo Square, Point Prometheus and then the big finale.

I believe that is correct - This game is pretty cool, just out of curiousity who rescued all the little sisters?

I'm on my way to doing so.

I just finished the Fort whatever stage and have got everyone so far.

The bonus you get for doing so is immense at this point.

I saved them all, but only because, in the end, you get more adam than by harvesting them* and you get the "good" ending.

Of course, I haven't put together any numbers myself, but what I've read on the "teh Internets" is in general accordance with my experience. Saving all the little buggers I had no trouble maxing out all my tracks and buying a wide variety of plasmids and such my first run through.

You get less Adam from saving them overall but you get other goodies including ammo, health/eve kits and plasmids. I beat the game already saving all of them and trying again on hard harvesting all of them. I heard there's a 3rd ending, going half and half with the Sisters. Can anyone confirm that?

I beat the game harvesting all of them. I actually wasn't able to buy everything at the Gatherer's Gardens though. I missed out on one Engineering Slot and several max Eve upgrades. I was practically full on everything else though.

I'll go through it again going 50/50 to check that out, Lokus.

Heh, well you don't have to do it on my behalf. I'm probably going to make a separate save halfway through and play again saving all the rest from there on in. I got to say though, the harvesting sequence is damn creepy and disturbing.

Edit: According to this thread on Gamefaqs, there's only two endings.

You do get less adam.... I didn't notice that. I mean, I knew that you received less when you rescued them, but I thought the gift from Tennenbaum made up the difference. Hmmm. :hmm:

Even rescuing the little darlings I didn't have any serious problem with the game on Normal. Like I said, maxed out slots, had ample goodies and such, which is odd because normally, I really stink at shooters on the console.

The game is much more difficult on hard. I just started playing the game and I am doing so on hard, I must say, it is rather tough. The game is glorious though.

YaWhateva, are you saying you are playing it your first time through on hard and it's hard? If so, how do you know how MUCH harder it is than medium? ;-)

I started it on medium and then restarted on hard because it wasn't very challenging. :)

The first time I played it I put it on hard difficulty. I didn't even bother with medium. I harvested them the first time and I kinda rushed through it. Now I'm playing it again and really soaking up the world (no pun intended) and rescuing the Little Sisters. I also somehow missed my last PttP station for one of my weapons and a few audio diaries. I'm going through it with an audio diary guide this time though.

I saved most of the little sister's until the lady gave me the reward and after that I just harvested them for more atom's. The ending was rather odd, so if everyone here is saying there are three endings, I better play it on normal this time.

Yeah, I played it on easy, but it will still hard, so now normal is going to be extremely difficult, but oh well. I liked having the frozen plasmid level 3, it helped when fighting Mr. Fontaine.

She gives you more than one reward, you know ;)

I think it's one reward every three saved Sisters

Now I have a second reason to play the game again.

As soon as I figured out it was a "slow" fps shooter like System Shock 2 where you are rewarded for looking into every crack and crevice, I have been enjoying the game immensely. The atmospheres are fantastic, and I love the art-deco theme throughout. I've just started Arcadia level and its pretty damn frightening!

Useless tip of the day: If you use telekinesis to grab a dead splicer and throw it at another splicer or enemy, they instantly die, except for the Big Daddy's, they just get stunned for two seconds.