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Ouch, I was gonna recommend Disk Management to reassign the drive letter but it looks like you've already tried it.
What I WOULD recommend is trying it on each PC with no other USB devices plugged in (if possible). Many USB devices like printers and hard drives will function very oddly if they don't have enough USB power. They will show up in Windows briefly and then disappear, etc. Try different USB slots on both PCs and if that doesn't work, unplug every USB device and try it alone.
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