You said you powered down the computer, then reseated the vid card. All modern motherboards still have like some power going on, unless you actually remove the power cord or use the PS power switch to turn it off. So, with power to the motherboard, you accidently removed the vid card. Then, again, with power still supplied to the motherboard, you reseated the vid card. Hmmmm... Probably not good. I think it's 3.3V all the time unless you use the kill switch on the PS or remove the power cord.
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