Tilted Forum Project Discussion Community - View Single Post - Extracting DNA from anything living. Is this for real?

hilbert25 · 03-15-2005, 08:27 PM

INAB (I'm not a biologist) but my SO is. Now the DNA 2.0 thing: That actually appears fairly common in science (there is a price wars of sorts going on right now, with pricing per the base pair). What happens, as I understand it, is you ask for a certain sequence, they make it, you then try to insert it into whatever you're trying to insert it into, so that you can express something or another, put a sequence for a glowing protein next to the sequence that creates a certain protein, and perhaps when that protein is expressed, the glowy one will be too, so that you can see which cells expressed it by counting the glowing ones. The hard part here is figuring out what will happen when you actually put the sequences together.

Now the part about extracting your DNA. It sounds a lot like what my SO does to extract DNA in her lab. You break apart the cells some how (in her case with tiny glass beads) and mix it around until the DNA comes out on top, that's the scientific explanation

. The use of a blender seems a little strange until you realize that blenders were invented in a bio lab. And the amount you get back is also plausible since my SO once reported a messing with a 2 foot long strand of DNA and cutting it with scissors.

03-15-2005, 08:27 PM	#15 (permalink)
hilbert25 Psycho Location: nOvA	INAB (I'm not a biologist) but my SO is. Now the DNA 2.0 thing: That actually appears fairly common in science (there is a price wars of sorts going on right now, with pricing per the base pair). What happens, as I understand it, is you ask for a certain sequence, they make it, you then try to insert it into whatever you're trying to insert it into, so that you can express something or another, put a sequence for a glowing protein next to the sequence that creates a certain protein, and perhaps when that protein is expressed, the glowy one will be too, so that you can see which cells expressed it by counting the glowing ones. The hard part here is figuring out what will happen when you actually put the sequences together. Now the part about extracting your DNA. It sounds a lot like what my SO does to extract DNA in her lab. You break apart the cells some how (in her case with tiny glass beads) and mix it around until the DNA comes out on top, that's the scientific explanation . The use of a blender seems a little strange until you realize that blenders were invented in a bio lab. And the amount you get back is also plausible since my SO once reported a messing with a 2 foot long strand of DNA and cutting it with scissors. Last edited by hilbert25; 03-15-2005 at 08:29 PM..