I'd check a lot of things before suspecting that the transmission is the culprit. Plus the problem MrSelfDestruct described would be more of a rapid surging than a vibration. BTW, does the steering wheel shake, or is it more something you feel in your butt?
I had a car that had a pretty bad vibration at highway speed when coasting in gear or accelerating. Shifting into neutral or giving it just the right amount of gas to maintain speed smoothed it out considerably. Turned out to be the front wheel bearings.
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