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I would say, NO, don't take time off. Instead, do the exercise on the lightest setting first, and moniter your heart rate. You want to start at around 65% (adjust to fit your age and needs). This might mean you take all the weights off the machine and just go through the motions but if you don't keep at it, you'll lose most of what you stood to gain from your body's pain.
This is, of course, based on your pain being muscular pain from simple overexertion. The good pain is a warm ache felt in the thickest part of the muscle. If you feel a sharp, stabbing pain, a tearing pain, or pain in a joint or tendon, you might have an injury. If you suspect this is the case you want to visit the doctor before going back to the gym lest you do yourself permanent harm.
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