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Old 12-10-2005, 12:41 PM   #36 (permalink)
rofgilead
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Location: Nowhere
Gel Preparation:

Mix 110 ml of a 2% ampholyte solution and add the appropriate detergent (Note: Concentration of ampholytes below 1% (w/v) often result in unstable pH gradients! Ampholytes at concentrations ABOVE 3% (w/v) are difficult to remove from gels - and - can interfere.. with protein.. staining!)
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